I recently tried to annotate two assembled metagenome samples derived from the same sample type (both samples come from the same type of material) using blastp and the metagene gene predictor tool (via WebMGA). I get a similar number of ORFs predictions for both samples. However after I input these ORF predictions into blastp (same settings for both samples) the number of blastp outputs for one sample were much higher than for the other sample. Why would I get more blastp hits for samples with a similar number of ORFs predicted?
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM