Hi,
I have such a problem. I had my MiSeq experiment where I was sequencing some amplicons which were dual indexed (each sample had 2 unique barcodes)
Something like this:
sample1 - AB
sample2 - CD
sample3 - EF
So we didnt have any AD, CF, AF... ect.
In the end, MiSeq could not demultiplex our reads because only one barcode was detected.
Something like this:
@HWI-M02221:13:000000000-A7W2J:1:1101:11982:1825 1:N:0:NGNNNCTNCGTCTAAT
ACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTACGAGTGTCGTGCAGCCTCCAGGCCCCCCCCTCCCGGGAGAGCCATAGTGGTCTTCGGAACCGGTGAGTACACCGGAATCGCTGGGGTGACCGGGTCCTTTCTTGGAACAACCCGCTCAATACCCAGAAATTTTGGCGTGCCCCCGCGAGATCACTAGCCGAGTATTGTTTGTTCCCCAAAGGCCTTGTGGTACTGCCTCATATNNTNCTTGGNATCTTAATATCACCCTTTNTGACTCTATNCCNCGAGGCTGGTCTCCN
@HWI-M02221:13:000000000-A7W2J:1:1101:19763:1825 1:N:0:NCNNNCTNCTACTGAC
ACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTACGAGTGTCGTGCAGCCTCCAGGACCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTGAGTACACCGGAATCGCTGGGTTGACCGGGTCCTTTCTTGGAACAACCCGCTCAATACCCAGAAATTTGGGCGTGCCCCCGCGATATCACTATCCTCGTAGTCTTGGGTCGCGAAACGCCTTTTGTTACTGCCTTATACNNTNCTTTANACCTTAATCTCACACTTTNTGACTCCATNCCNACTCGCTAACCTCCN
Do you know any method to demulitpex such kind of data, considering that even with one barcode I know from which sample originates my amplicon. Any software, sugestions?
I have such a problem. I had my MiSeq experiment where I was sequencing some amplicons which were dual indexed (each sample had 2 unique barcodes)
Something like this:
sample1 - AB
sample2 - CD
sample3 - EF
So we didnt have any AD, CF, AF... ect.
In the end, MiSeq could not demultiplex our reads because only one barcode was detected.
Something like this:
@HWI-M02221:13:000000000-A7W2J:1:1101:11982:1825 1:N:0:NGNNNCTNCGTCTAAT
ACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTACGAGTGTCGTGCAGCCTCCAGGCCCCCCCCTCCCGGGAGAGCCATAGTGGTCTTCGGAACCGGTGAGTACACCGGAATCGCTGGGGTGACCGGGTCCTTTCTTGGAACAACCCGCTCAATACCCAGAAATTTTGGCGTGCCCCCGCGAGATCACTAGCCGAGTATTGTTTGTTCCCCAAAGGCCTTGTGGTACTGCCTCATATNNTNCTTGGNATCTTAATATCACCCTTTNTGACTCTATNCCNCGAGGCTGGTCTCCN
@HWI-M02221:13:000000000-A7W2J:1:1101:19763:1825 1:N:0:NCNNNCTNCTACTGAC
ACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTACGAGTGTCGTGCAGCCTCCAGGACCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTGAGTACACCGGAATCGCTGGGTTGACCGGGTCCTTTCTTGGAACAACCCGCTCAATACCCAGAAATTTGGGCGTGCCCCCGCGATATCACTATCCTCGTAGTCTTGGGTCGCGAAACGCCTTTTGTTACTGCCTTATACNNTNCTTTANACCTTAATCTCACACTTTNTGACTCCATNCCNACTCGCTAACCTCCN
Do you know any method to demulitpex such kind of data, considering that even with one barcode I know from which sample originates my amplicon. Any software, sugestions?
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