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Hi everyone, I am little confused Cigar and cigar-op. Can you explain the diff? Thank you!
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a cigar string is an array of cigar elements
a cigar element is a pair of cigar-operator, cigar-length
cigar-operator (H,S,E,X,=,I,D...) define the type of pairwise alignment (e.g D=deletion from REF)
cigar-length is the length of the operation (e;g: D100 deletion of 100bp) -
Can you give me the meaning of all those signals H,S,E,X,=, I,D,.. or which material I can look up them?Originally posted by lindenb View PostComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
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The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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