Was there an error? Post that.
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Finally got it .heartly thankyou to all
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anybody have good and new version manual send to meComment
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I do not think they got an error. When I run the code I get back the qcovs value and its a number value. I think that they are asking for the hsp_middleline value that shows where the protein and matches and doesn't. The only way I know how to get that is in the xml format and then you either have to cut and paste it from there or have a scritp to extract it from the file. Which is out of m relm of ability, Maybe GenoMax knows how to do that from the standalone BLAST but I have not done it before, other then just using the xml format option.Comment
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Command line manual from NCBI is linked in post #4.Originally posted by kulandaisamy View PostComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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