We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained.
One example file has been attached.
We are now blasting each of the novel miRNA sequence against the miRBase which has yielded miRNA sequences from other animal species like human, mice etc (E-value as small as 0.0001). We are then opening:
1. Interaction explorer (http://www.informatics.jax.org/interaction/explorer)*
2. Gene Ontology Classifier
3. KEGG
Please advice what should be the pipeline to annotate the novel miRNA*in a species like buffalo where Chromosome wise genomic data are not available?
How to proceed for Annotation and Characterization of novel miRNA?.
One example file has been attached.
We are now blasting each of the novel miRNA sequence against the miRBase which has yielded miRNA sequences from other animal species like human, mice etc (E-value as small as 0.0001). We are then opening:
1. Interaction explorer (http://www.informatics.jax.org/interaction/explorer)*
2. Gene Ontology Classifier
3. KEGG
Please advice what should be the pipeline to annotate the novel miRNA*in a species like buffalo where Chromosome wise genomic data are not available?
How to proceed for Annotation and Characterization of novel miRNA?.
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