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  • how can I retrieve one read sequence?

    Hi, I use Integrative genomic Viewer to find one Read which mate is mapped=no.
    This no mapped pair-end read may be a result from structural variation. So, if I know one read ID and its one end sequence, How can I get its pair-end sequence from raw Data.

    Thanks!

  • #2
    If your sequence files are not compressed:

    Code:
    $ cat file.fastq | grep -A 3 "read_ID_you_want"
    If they are compressed:

    Code:
    $ zcat file.fastq.gz | grep -A 3 "read_ID_you_want"
    If you only want sequence (not full fastq record) then replace 3 with 1.

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