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  • Rahul shelke
    Junior Member
    • Mar 2015
    • 8

    #1

    Velvet assembler and scaffold

    hello,
    My contigs.fa file generated from velvet assembly contains NNNNNNN base in every sequences.
    so does it mean that my contigs.fa file is scaffold file or only assembly file?
    If not can I generate good scaffolds by using velvet.....?

    used command....
    for velvetg
    velvetg out_data_29 -clean yes -exp_cov 21 -cov_cutoff
    2.81 -min_contig_lgth 200


    Thanks
  • paa6
    Member
    • Feb 2014
    • 68

    #2
    in my opinion NNNNNNN shows gaps. may be u need to check quality of ur reads before going for assembly. try FastQC.

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