Contigs assembly by DNASTAR

MZB

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Join Date: Apr 2015

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Contigs assembly by DNASTAR

04-03-2015, 10:09 AM

Hello Everyone, I am working on NGS and I have done De novo assembly for one KIR locus using DNASTAR SeqMan NGen. I got so many contigs more than 1000pb and I am interested on all KIR genes. That's not helpul when it comes to the alignment and analysis. My question is: what assembly parameters should I change first to make the contig assembler construct longer contigs, and which parameters should I avoid at the risk of introducing major errors? I do not know what to do with the sequences of each contigs. Should I just choose one contigs of all of contigs generated for the alignement? Does anyone can give me a short worklow for that?
Thank u
Tags: assembly contigs dnastar

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