Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • MZB
    Junior Member
    • Apr 2015
    • 3
    #1

    Contigs assembly by DNASTAR

    Hello Everyone, I am working on NGS and I have done De novo assembly for one KIR locus using DNASTAR SeqMan NGen. I got so many contigs more than 1000pb and I am interested on all KIR genes. That's not helpul when it comes to the alignment and analysis. My question is: what assembly parameters should I change first to make the contig assembler construct longer contigs, and which parameters should I avoid at the risk of introducing major errors? I do not know what to do with the sequences of each contigs. Should I just choose one contigs of all of contigs generated for the alignement? Does anyone can give me a short worklow for that?
    Thank u
    Tags: assembly contigs dnastar
    Previous template Next

    Latest Articles

    Collapse
    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Gene Copies Once Dismissed as Junk DNA Shape Animal Adaptation and Human Disease by SEQadmin2
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    26 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    11-12-2026, 07:59 AM  
    WashU Medicine Study Verifies Methods for Tracking Genetic Mosaicism by SEQadmin2
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    11-05-2026, 09:25 AM  
    HuBMAP Portal Offers Free Access to Single-Cell Data from Healthy Human Tissues by SEQadmin2
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    55 views
    0 reactions
    		 Last Post kinpict.com
    by kinpict.com
    Today, 06:49 PM  
    Genome Sequencing Reveals Large, Repeat-Rich DNA in Trapdoor Spider Named for Stephen Colbert by SEQadmin2
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    10-28-2026, 09:57 AM  
    View All
    Working...