I'm sure this has been asked before, but does anyone know how to perform upper quartile normalization on RNA-seq data? I cannot find a script to do this and it seems like it should be a simple matter of getting the read counts for each lane and then normalizing (but I'm not certain as to the statistical calculation behind this, although I know it is not complex). Thanks.
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anyone? a little help here would be much appreciated. -
Goby supports Upper Quartile normalization since version 1.5. See argument --normalization-methods at http://campagnelab.org/software/goby...tation-counts/
See also http://campagnelab.org/software/goby...al-expression/Comment
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