I'm sure this has been asked before, but does anyone know how to perform upper quartile normalization on RNA-seq data? I cannot find a script to do this and it seems like it should be a simple matter of getting the read counts for each lane and then normalizing (but I'm not certain as to the statistical calculation behind this, although I know it is not complex). Thanks.
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anyone? a little help here would be much appreciated. -
Goby supports Upper Quartile normalization since version 1.5. See argument --normalization-methods at http://campagnelab.org/software/goby...tation-counts/
See also http://campagnelab.org/software/goby...al-expression/Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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