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My guess is the sequence has been reverse complemented, and that's why you can't find it. As dpryan suggests, try searching by the read name instead.
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Try grepping for the read name, that's less error prone:
Code:zgrep -A 3 "HWI:stuff" file.fastq.gz
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why the read sequence from IGV can't be found in fastq?
I found a strange read pair from IGV, so i want to extract the original information about the read pair.
I use " zcat file.fastq.gz | grep -B 3 "TCTCAAAAAGCACATGGATTCTACTCCTGATTTCTCCAAGGATCTACATATGATCAAAACATATCTTTACCAGATTCTCCGTGGAATTGCGTATTGCCACTCTCATAGGGTTCTCCATCGTGATC"
The strange thing is i can't find it in original fq file.Tags: None
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by SEQadmin2
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...-
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