I have been given some GFF files to process, and one of these is throwing an error when I attempt to run it through HTSeq-count:
Error occured when processing GFF file (line 26 of file ./features/promoter.gff):
Cannot subset to zero-length interval.
[Exception type: IndexError, raised in _HTSeq.pyx:348]
Indeed, on line 26, the start value is higher than the end value for the interval.
Has anyone encountered this before?
Should I trust the quality of this GFF file or is something terribly wrong?
How would you deal with this problem?
Thanks in advance...
Error occured when processing GFF file (line 26 of file ./features/promoter.gff):
Cannot subset to zero-length interval.
[Exception type: IndexError, raised in _HTSeq.pyx:348]
Indeed, on line 26, the start value is higher than the end value for the interval.
Code:
C20524 flankbed promoter 1101 1100 . - . ID=CGI_10000033;
Should I trust the quality of this GFF file or is something terribly wrong?
How would you deal with this problem?
Thanks in advance...
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