Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • sylvie.le.borgne
    Junior Member
    • Apr 2015
    • 3

    Install ea-utils

    Hi, I am new to Bioinformatics and Linux, I have dowloaded ea-utils 1.1.2-537 in my Ubuntu box and unzipped it. I tried to use the fastq-join with my paired reads but the following message appeared:
    fastq-joint command not found
    Is there something else i need to do to install ea-utils in my box?
    Thank you
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    I don't recommend fastq-join; in my tests, it's worse than pretty much every alternative other than Pear.

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      Originally posted by sylvie.le.borgne View Post
      Hi, I am new to Bioinformatics and Linux, I have dowloaded ea-utils 1.1.2-537 in my Ubuntu box and unzipped it. I tried to use the fastq-join with my paired reads but the following message appeared:
      fastq-joint command not found
      Is there something else i need to do to install ea-utils in my box?
      Thank you
      Probably not (assuming ea-utils are already compiled). You may need to add execute permissions

      Code:
      $ chmod u+x /path_to/fastq-join
      or provide full path to fastq-join as you try to run it

      Code:
      $ /path_to/fastq-join your_R1_file your_R2_file

      Comment

      • sylvie.le.borgne
        Junior Member
        • Apr 2015
        • 3

        #4
        Thank you, I tried but it did not work. In the ea-utils folder I have fastq-join.c, I dont know what is the meaning of the letter c....

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          Originally posted by sylvie.le.borgne View Post
          Thank you, I tried but it did not work. In the ea-utils folder I have fastq-join.c, I dont know what is the meaning of the letter c....
          That means it is source code that needs to be compiled. There should be a readme-type document included in the source to tell you how to compile the code to make executable files.

          Generally a
          Code:
          $ make
          in the directory may be all that is needed.

          Comment

          • sylvie.le.borgne
            Junior Member
            • Apr 2015
            • 3

            #6
            you mean this:

            make home/sylvie/desktop/ea-utils.1.1.2-537/

            or make home/sylvie/desktop/ea-utils.1.1.2-537 ?

            Comment

            • GenoMax
              Senior Member
              • Feb 2008
              • 7142

              #7
              Code:
              $ cd /home/sylvie/desktop/ea-utils.1.1.2-537
              $ make
              Good guide for basics of unix for a biologist: http://korflab.ucdavis.edu/Unix_and_...ent.html#part1

              Comment

              • LVAndrews
                Member
                • Sep 2012
                • 55

                #8
                fastq-join works OK, you just need to relax it because it is so stringent. I use -m 30 -p 30 with good success. Some data is crappier than other data and may need additional grooming before it can be successfully merged.

                Comment

                • bio_informatics
                  Senior Member
                  • Nov 2013
                  • 182

                  #9
                  Late here:
                  Last I recall installing, ea-utils needed libgsl-dev library to install
                  Bioinformaticscally calm

                  Comment

                  Latest Articles

                  Collapse

                  • SEQadmin2
                    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
                    by SEQadmin2


                    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


                    Here are nine questions we think about, in roughly the order they matter, before...
                    06-18-2026, 07:11 AM
                  • SEQadmin2
                    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
                    by SEQadmin2


                    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


                    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
                    ...
                    06-02-2026, 10:05 AM

                  ad_right_rmr

                  Collapse

                  News

                  Collapse

                  Topics Statistics Last Post
                  Started by SEQadmin2, 06-17-2026, 06:09 AM
                  0 responses
                  25 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 06-09-2026, 11:58 AM
                  0 responses
                  42 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 06-05-2026, 10:09 AM
                  0 responses
                  48 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 06-04-2026, 08:59 AM
                  0 responses
                  49 views
                  0 reactions
                  Last Post SEQadmin2  
                  Working...