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  • FelipeDre
    Junior Member
    • Apr 2015
    • 1

    #1

    FastQC per sequence gc one sharp peak and one broad

    I sequenced human data and my fastqc records fail in the "per sequence gc content" where a very sharp peak appears and a broad second. The sharp peak centres at 51%. Could this be contamination (since human gc content is expected at 42%)?
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  • AntonioRFranco
    Member
    • Feb 2013
    • 22

    #2
    It you access the help, you can read it can be attributed to many causes. Contamination of your samples can be one, but not the only

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