Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • colibri14
    Junior Member
    • Nov 2014
    • 1

    #1

    Trinity error in both.fa

    Hi!

    I am using Trinity (r2014-07-17) for de novo assembly of RNA-seq data (100bp PE). I have 12 samples and 9 of them have successfully completed, however 3 fail early on in the program.

    For these samples, the error message I get is:
    HTML Code:
    Monday, April 13, 2015: 15:18:53	CMD: mkdir -p /brashear/mlim/Trinityfiles/trinityResults/CGML001B/chrysalis
    CMD finished (0 seconds)
    Converting input files. (in parallel)Monday, April 13, 2015: 15:18:54	CMD: /usr/local/packages/trinity/r2014-07-17/trinity-plugins/fastool/fastool --illumina-trinity --to-fasta /brashear/mlim/Trinityfiles/CGML001B_trinity_left.fq >> left.fa 2> /brashear/mlim/Trinityfiles/CGML001B_trinity_left.fq.readcount 
    Monday, April 13, 2015: 15:18:54	CMD: /usr/local/packages/trinity/r2014-07-17/trinity-plugins/fastool/fastool --illumina-trinity --to-fasta /brashear/mlim/Trinityfiles/CGML001B_trinity_right.fq >> right.fa 2> /brashear/mlim/Trinityfiles/CGML001B_trinity_right.fq.readcount 
    CMD finished (62 seconds)
    Thread 1 terminated abnormally: Error, cmd: /usr/local/packages/trinity/r2014-07-17/trinity-plugins/fastool/fastool --illumina-trinity --to-fasta /brashear/mlim/Trinityfiles/CGML001B_trinity_left.fq >> left.fa 2> /brashear/mlim/Trinityfiles/CGML001B_trinity_left.fq.readcount  died with ret 256 at /usr/local/packages/trinity/r2014-07-17/Trinity line 1990.
    Use of uninitialized value in array dereference at /usr/local/packages/trinity/r2014-07-17/Trinity line 1171.
    Error prepping sequences. at /usr/local/packages/trinity/r2014-07-17/Trinity line 1175.
    For reference, when it works, I get:
    HTML Code:
    Wednesday, April 8, 2015: 18:40:33	CMD: mkdir -p /brashear/mlim/Trinityfiles/trinityResults/CGML001A/chrysalis
    CMD finished (0 seconds)
    Converting input files. (in parallel)Wednesday, April 8, 2015: 18:40:33	CMD: /usr/local/packages/trinity/r2014-07-17/trinity-plugins/fastool/fastool --illumina-trinity --to-fasta /brashear/mlim/Trinityfiles/CGML001A_trinity_left.fq >> left.fa 2> /brashear/mlim/Trinityfiles/CGML001A_trinity_left.fq.readcount 
    Wednesday, April 8, 2015: 18:40:33	CMD: /usr/local/packages/trinity/r2014-07-17/trinity-plugins/fastool/fastool --illumina-trinity --to-fasta /brashear/mlim/Trinityfiles/CGML001A_trinity_right.fq >> right.fa 2> /brashear/mlim/Trinityfiles/CGML001A_trinity_right.fq.readcount 
    CMD finished (47 seconds)
    CMD finished (49 seconds)
    Done converting input files.Wednesday, April 8, 2015: 18:41:22	CMD: cat left.fa right.fa > both.fa
    CMD finished (21 seconds)
    Has anyone gotten these errors before? I'd appreciate any suggestions!
    Thanks!!
  • enelkinsan
    Member
    • May 2012
    • 14

    #2
    This loos like input problem. Check if your fastq files for 3 fails are in correct format.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    30 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    39 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Working...