Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • fanli
    Senior Member
    • Jul 2014
    • 197

    #1

    Tophat gtf_to_fasta ignoring strand

    Hi all,

    Am I crazy, or does Tophat's gtf_to_fasta utility seem to ignore strand information? Using the hg19 iGenomes release, for example. Transcript NR_024540 is on the minus strand.

    Code:
    585	NR_024540	chr1	-	14361	29370	29370	29370	11	14361,14969,15795,16606,16857,17232,17605,17914,18267,24737,29320,	14829,15038,15947,16765,17055,17368,17742,18061,18366,24891,29370,	0	WASH7P	unk	unk	-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,
    Now, looking at the sequence in UCSC (first two lines):
    Code:
    >NR_024540.1
    tccggcagagcggaagcggcggcgggagcttccgggagggcggctcgcag
    gcaccatgactcctgtgaggatgcagcactccctggcaggtcagacctat
    Looking at the sequence from the genes.fa file that gtf_to_fasta outputs:
    Code:
    >1 NR_024540 chr1- 14362-14829,14970-15038,15796-15947,16607-16765,16858-17055,17233-17368,17606-17742,17915-18061,18268-18366,24738-24891,29321-29370
    TCCTGCACAGCTAGAGATCCTTTATTAAAAGCACACTGTTGGTTTCTGCTCAGTTCTTTA
    TTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTG
    # nope not the same!
    If I reverse complement the whole sequence and then look at the first two lines:
    Code:
    TCCGGCAGAGCGGAAGCGGCGGCGGGAGCTTCCGGGAGGGCGGCTCGCAG
    GCACCATGACTCCTGTGAGGATGCAGCACTCCCTGGCAGGTCAGACCTAT
    # yay this seems to match!

    Now, I sort of remember seeing this a while back, but forget what became of it. Anyone have insight? Is this a bug?

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 11:13 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, Yesterday, 10:48 AM
0 responses
18 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...