Hello everybody,
I'm new in NGS area and I would like to solve some questions.
One of them is:
I have many NGS sequences in a BAM format:
For example, for a same sequence, I have:
4M1D1M1D4M2I2M114S
CTTCCCCCACCACCGTTGGCACAGCGCCCCCGGGAACACCCTCCCACCACCACCGTCGGCACAGCGCCCCGGGGAGCACCCCAGCCCAGCTGCACCAGGGCTCTCTGAAGGAGGTGGTGGTCCGGTT
CTTC-C-CCCAAC
I don't understand why a great part of sequence was missed when it was converted CIGAR to aligned sequence. Can anyone help me?
Other question is: which sequence I use to convert it into an amino acid sequence?
CTTCCCCCACCACCGTTGGCACAGCGCCCCCGGGAACACCCTCCCACCACCACCGTCGGCACAGCGCCCCGGGGAGCACCCCAGCCCAGCTGCACCAGGGCTCTCTGAAGGAGGTGGTGGTCCGGTT or CTTC-C-CCCAAC?
In fact, I want to compare amino acids mutations in relation to the reference sequence.
Sorry for my english and thanks so much for the answers.
I'm new in NGS area and I would like to solve some questions.
One of them is:
I have many NGS sequences in a BAM format:
For example, for a same sequence, I have:
4M1D1M1D4M2I2M114S
CTTCCCCCACCACCGTTGGCACAGCGCCCCCGGGAACACCCTCCCACCACCACCGTCGGCACAGCGCCCCGGGGAGCACCCCAGCCCAGCTGCACCAGGGCTCTCTGAAGGAGGTGGTGGTCCGGTT
CTTC-C-CCCAAC
I don't understand why a great part of sequence was missed when it was converted CIGAR to aligned sequence. Can anyone help me?
Other question is: which sequence I use to convert it into an amino acid sequence?
CTTCCCCCACCACCGTTGGCACAGCGCCCCCGGGAACACCCTCCCACCACCACCGTCGGCACAGCGCCCCGGGGAGCACCCCAGCCCAGCTGCACCAGGGCTCTCTGAAGGAGGTGGTGGTCCGGTT or CTTC-C-CCCAAC?
In fact, I want to compare amino acids mutations in relation to the reference sequence.
Sorry for my english and thanks so much for the answers.
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