Hello
I'm having trouble to run rRNASelector on unix server through ssh.
It starts, the dialog box opens on my screen, I choose the reads file (fasta), but then I get this error message:
"Starting hmmsearch for forward using lib/16s_bact_for3.hmm
Not right file format! -> Abort"
My hmmer is ok, when I type hmmsearch -h it gives to me the help information.
I've tried to run it choosing all possible paths for hmmsearch, but it just crashes. Besides this, the dialog box dynamics is so slow, I cant either change the length parameter...
In parallel, I'm running Metaxa, and it seems to work well, but for my metagenomic dataset it is finding too few sequences. Is a poor richness dataset, in fact, but even though I was expecting more. My output for bacteria sequences is 61 reads of a total of 110504 sequences. This sequences are from Illumina paired end, merged through Flash, with a average combine of 81% and average length of 293
The sequences used for rRNASelector are the same
My goal is to run a diversity workflow in qiime.
Thanks for every suggestion!!!
I'm having trouble to run rRNASelector on unix server through ssh.
It starts, the dialog box opens on my screen, I choose the reads file (fasta), but then I get this error message:
"Starting hmmsearch for forward using lib/16s_bact_for3.hmm
Not right file format! -> Abort"
My hmmer is ok, when I type hmmsearch -h it gives to me the help information.
I've tried to run it choosing all possible paths for hmmsearch, but it just crashes. Besides this, the dialog box dynamics is so slow, I cant either change the length parameter...
In parallel, I'm running Metaxa, and it seems to work well, but for my metagenomic dataset it is finding too few sequences. Is a poor richness dataset, in fact, but even though I was expecting more. My output for bacteria sequences is 61 reads of a total of 110504 sequences. This sequences are from Illumina paired end, merged through Flash, with a average combine of 81% and average length of 293
The sequences used for rRNASelector are the same
My goal is to run a diversity workflow in qiime.
Thanks for every suggestion!!!