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  • BaySeq getPriors problem

    Hi all,
    I'm just starting to work with baySeq (switching from DESeq2 to see what's different), and I've run into a problem pretty early on with getPriors

    > head(all)
    S1IP_1 S1IP_2 S1IP_3 S2IP_1 S2IP_2 S2IP_3
    [1,] 93 35 92 271 231 350
    [2,] 2 3 7 9 7 10
    [3,] 0 5 1 0 0 1
    [4,] 1 14 9 3 5 0
    [5,] 0 0 0 0 1 0
    [6,] 5 98 15 17 19 48
    > seglens <- input [,5]
    > replicates <-c("S1","S1","S1","S2","S2","S2")
    > groups <- list(NDE = c(1,1,1,1,1,1), DE = c(1,1,1,2,2,2))
    > CD <- new("countData", data = all, seglens = seglens, replicates = replicates, groups = groups)
    > CD@annotation <- as.data.frame(cname)
    > libsizes(CD) <- getLibsizes(CD)
    >CDpriors <- getPriors.NB(CD, samplesize = 10^5, estimation = "QL", cl=cl)
    Finding priors...Error in checkForRemoteErrors(val) :
    one node produced an error: 'xmin' not less than 'xmax'


    Any ideas about what's going on and how I can fix it?
    Last edited by virg4l; 06-03-2015, 03:52 PM.

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