Hi,
I am still quite a noob at this, so bear with me.
I have some RNA Seq PE-50 data and I am trying to do some adapter trimming. Library was made with a Smarter Race Kit. I was thinking to remove the below adapter form mate 1 and its complementary from mater 2. Curiously, I also find reverse complementary adapters in my mate 1. I have been trying to look at the amplification flow chart but cannot figure how this could happen. Thankful for any hints that could help me understand.
Also, I was expecting the adapter close to the 5' end but can see it over the entire read span. What am I thinking wrong here?
Thanks,
I am still quite a noob at this, so bear with me.
I have some RNA Seq PE-50 data and I am trying to do some adapter trimming. Library was made with a Smarter Race Kit. I was thinking to remove the below adapter form mate 1 and its complementary from mater 2. Curiously, I also find reverse complementary adapters in my mate 1. I have been trying to look at the amplification flow chart but cannot figure how this could happen. Thankful for any hints that could help me understand.
Also, I was expecting the adapter close to the 5' end but can see it over the entire read span. What am I thinking wrong here?
Thanks,
Code:
Adapter AAGCAGTGGTATCAACGCAGAGTAC
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