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Is there a command that might help to transfer a BAM file to a website? Thank you 
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What sort of transfer? Uploading a bam file to a tool/site (e.g. galaxy) or uploading it to a web location so it becomes available for others to download.
For the latter, you would need to be able to access the backend server (so you can upload the file via scp/sftp). You will need an account on the server in question. -
It is the latter of the two (transfer to site to download from). Would lftp work?
3 files are put in a folder named "test" (BAM ~20GB, VCF, and coverage analysis) then zipped using:
Code:tar -zcf /path/to/file.tar.gz -C /Desktop/test
To view the transferCode:lftp https://something.sharefile.com/login.aspx lftp https://something.sharefile.com/login.aspx:~> user xxxxxx Password: xxxxxxx lftp https://something.sharefile.com/login.aspx:~> mirror -R /home/dnascopeV/Desktop/Test
To unzip (not sure if this will work as it might be basic HTTP and not what I need):Code:lftp https://something.sharefile.com/login.aspx:~> ls
ThanksCode:wget --http-user cmccabe --http -passwd xxxx*** https://something.sharefile.com/login.aspx -O - | tar -zxf -
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Looks like you have everything under control :-)
I would just download (reversing lftp) and then unzip locally to be safe after the transfer is complete.Comment
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can lftp be used in cygwin? Thank you.
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If you can compile it then yes. Give it a try.Comment
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I am not sure what you mean by downloading (reversing lftp), can you use it in an example. I just learned lftp a few weeks ago and finding it very useful. Thank you.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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