Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • How to find nonhomologous DNA sequences between 2 speices

    I want to indentify a non-human species(B ,the genome size is about 100Mb) from the mixture of human and B species by target sequecing method.
    so the first thing is to find the nonhomologous DNA sequences between two species(human and B species) from genome.fa file ,and generate the *.fa file which includes the sequeces specifically in B species but no in human.
    So can anyone introduce me a software to find nonhomologous DNA sequences between them?
    Thanks!

  • #2
    Originally posted by lwgcc View Post
    I want to indentify a non-human species(B ,the genome size is about 100Mb) from the mixture of human and B species by target sequecing method.
    so the first thing is to find the nonhomologous DNA sequences between two species(human and B species) from genome.fa file ,and generate the *.fa file which includes the sequeces specifically in B species but no in human.
    So can anyone introduce me a software to find nonhomologous DNA sequences between them?
    Thanks!
    For this initial analysis have you considered using BLAT? What is the status of genome sequence for species B (contigs, chromosomes)? Is species B related to humans?

    BBSplit.sh from BBMap suite would be ideal when you are actually ready to assign NGS sequences to individual genomes.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    66 views
    0 likes
    Last Post seqadmin  
    Working...
    X