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  • mbk0asis
    Member
    • Dec 2011
    • 41

    #1

    DEXSeq error - Error in lf[[1]] : subscript out of bounds

    Hi,

    I was going to try DEXSeq for the splicing analysis, but I got stuck at the beginning.
    I got 'subscript out of bounds' errors when tried 'DEXSeqDataSetFromHTSeq'. The error seems to tell me that sample or column numbers were not matching with something else.
    I don't really understand it. Please, somebody tell me what did I do wrong?

    Code:
    library("DEXSeq")
    
    sampleTable <- data.frame(
        rownames = c( "IVF1","IVF2","IVF3",
                      "cSCNT1","cSCNT2","cSCNT3","cSCNT4",
                      "fSCNT1","fSCNT2","fSCNT3","fSCNT4"),
        countFiles = c( "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/IVF1.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/IVF2.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/IVF3.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/cSCNT1.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/cSCNT2.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/cSCNT3.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/cSCNT4.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/fSCNT1.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/fSCNT2.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/fSCNT3.counts",
                       "~/00-NGS/RNAseq/bov/Old_Pico/Ensembl/DEXseq/fSCNT4.counts"),
        condition = c( rep("IVF", 3), rep("cSCNT", 4),rep("fSCNT", 4) ),
        type = c("single","single","single","single","single","single","single","single","single","single","single"))
    sampleTable
    
    flattenedfile="/home/bio1/00-NGS/RNAseq/bov/Old_Pico/Annotation/DEXseq_Bovine_Ensembl.gtf"
    
    dxd = DEXSeqDataSetFromHTSeq(
          countFiles,
          sampleData=sampleTable,
          design= ~ sample + exon + condition:exon,
          flattenedfile=flattenedfile)

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