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  • metheuse
    Member
    • Jan 2013
    • 84

    #1

    BWA properly paired reads are not really properly paired?

    I selected properly paired (and mapq>=4) reads from BWA alignment by running:
    samtools view -h -f 2 -q 4 -b
    Flagstat shows that this did remove most of the reads that have mates on different chr. However, several remain:
    5 + 0 with mate mapped to a different chr
    4 + 0 with mate mapped to a different chr (mapQ>=5)
    Why is this?
    Thanks.

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