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  • AnushaC
    Member
    • Sep 2013
    • 78

    #1

    error snpEff annotated VCF hwe(****)

    Dear All,

    I am trying to annotate my vcf file using java -jar SnpSift.jar hwe
    achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe
    Usage: java -jar SnpSift.jar hwe [-v] [-q] [file.vcf]
    -q : Be quite
    -v : Be verbose
    achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe /mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf
    00:00:00.000 Reading '/mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf'. Running single threaded mode.
    Exception in thread "main" java.lang.NullPointerException
    at ca.mcgill.mcb.pcingola.vcf.VcfEntry.getGenotypesScores(VcfEntry.java:352)
    at ca.mcgill.mcb.pcingola.snpSift.hwe.VcfHwe.hwe(VcfHwe.java:42)
    at ca.mcgill.mcb.pcingola.snpSift.hwe.SnpSiftCmdHwe.run(SnpSiftCmdHwe.java:93)
    at ca.mcgill.mcb.pcingola.snpSift.SnpSift.run(SnpSift.java:364)
    at ca.mcgill.mcb.pcingola.snpSift.SnpSift.main(SnpSift.java:69)
    I dont know why I am getting this error and I could run every other SnpSift commands

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