Dear All,
I am trying to annotate my vcf file using java -jar SnpSift.jar hwe
achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe
Usage: java -jar SnpSift.jar hwe [-v] [-q] [file.vcf]
-q : Be quite
-v : Be verbose
achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe /mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf
00:00:00.000 Reading '/mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf'. Running single threaded mode.
Exception in thread "main" java.lang.NullPointerException
at ca.mcgill.mcb.pcingola.vcf.VcfEntry.getGenotypesScores(VcfEntry.java:352)
at ca.mcgill.mcb.pcingola.snpSift.hwe.VcfHwe.hwe(VcfHwe.java:42)
at ca.mcgill.mcb.pcingola.snpSift.hwe.SnpSiftCmdHwe.run(SnpSiftCmdHwe.java:93)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.run(SnpSift.java:364)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.main(SnpSift.java:69)
I dont know why I am getting this error and I could run every other SnpSift commands
I am trying to annotate my vcf file using java -jar SnpSift.jar hwe
achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe
Usage: java -jar SnpSift.jar hwe [-v] [-q] [file.vcf]
-q : Be quite
-v : Be verbose
achimmiri@idash-cloud-979:/mnt/oncogxB/anusha/snpEff$ java -jar SnpSift.jar hwe /mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf
00:00:00.000 Reading '/mnt/oncogxB/anusha/brain/brain.TCMOSAIC_BQ20.vcf'. Running single threaded mode.
Exception in thread "main" java.lang.NullPointerException
at ca.mcgill.mcb.pcingola.vcf.VcfEntry.getGenotypesScores(VcfEntry.java:352)
at ca.mcgill.mcb.pcingola.snpSift.hwe.VcfHwe.hwe(VcfHwe.java:42)
at ca.mcgill.mcb.pcingola.snpSift.hwe.SnpSiftCmdHwe.run(SnpSiftCmdHwe.java:93)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.run(SnpSift.java:364)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.main(SnpSift.java:69)
I dont know why I am getting this error and I could run every other SnpSift commands