Cuffdiff output to GSEA

[yaz1992]

Hi all,
I'm a beginner in Bioinformatics and I'm now work on some RNA-seq results. I'm here looking for help in GSEA analysis and any advice will be appreciated

I went through the Tophat-cufflinks process and now I want to use the cuffdiff output to GSEA. It says that four types of files should be prepared for GSEA. For the gene set file, I used the MsigDB, and I used Fpkm_trackingToGct module from gene pattern to get gct file from my cuffdiff output. However, I'm not sure if there is any module or software I can use to get the cls and chip annotation file from the cuffdiff output files. Or if I need to make them by myself, the cls file might be easy but I'm not sure about the annotation file, and I'm not sure which chip file I should use from the broad ftp web site.

Thanks in advance for helping!
Andrea

[Yena]

Hi Andrea,

Did you find a solution to this by any chance? I am currently stuck there as well, and I would really appreciate any guidance..

Thank you,
Yena