Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Unknown Exome capture kit dataset ncbi

    Hi Guys

    I want to do coverage analysis for the exome data (downloaded from NCBI SRR1299147). In this regard, I want to know what exome capture kit they have used, but i could not find any information in meta data as well. Is there any way to find that? if not what should i do and how should i proceed?

    what i am interested in, is to have bed file containing the target and probes, so that i can use bedtools(coverage command) to find the sequencing depth.

    A general question is, if some one gives a data set with no information about the exome capture kit. how should one proceed with the coverage analysis using bed tools? Any help in the right direction is highly appreciated. Thank you.

  • #2
    Looks like SureSelect Human All Exon 50Mb kit (Agilent Technologies) based on the linked publication: http://www.ncbi.nlm.nih.gov/Traces/sra/?study=SRP042250 and http://www.ncbi.nlm.nih.gov/pubmed/25987131

    Comment


    • #3
      @GenoMax. Thanks for your reply. i found the corresponding(SureSelect Human All Exon 50Mb kit) bed file from Agilent.

      A general question is, if some one gives a data set with no information about the exome capture kit. how should one proceed with the coverage analysis? Thank you.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      31 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      33 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      53 views
      0 likes
      Last Post seqadmin  
      Working...
      X