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I didn't see this posted, and it may be of interest. One thing to note is that all the aligners were run using defaults. Not sure if that is appropriate for these comparisons:
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Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com -
I saw that... though I'm not really sure what the comparison means, or how to evaluate which aligner is correct. -
Maybe the whole thing should just be considered a demo of the anvi'o browser/visualizer more than a rigorous comparison. The reference is a de novo microbiome assembly, which is an extra complication.
It did catch my eye, because I've used Novoalign before and thought it gave good results, and have been using BBMap and think it is excellent, and both are grouped in the "overmapper" bin of aligners. They also share the ability to allow many mismatches as well. So given a diverse source material which was condensed to a reference that has a single dominant representation of any related sequence, I'm not surprised that aligners that allow only a few mismatches wouldn't map as many, but was surprised that being able to map was considered an error.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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