Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Sciurus
    Member
    • Dec 2013
    • 23

    #1

    Cuffdiff error???

    Hi everybody,

    I keep getting the same error when trying to run cuffdiff:
    number of labels must match number of conditions.

    I have two groups/ treatments with 4 replicates each.

    Now, I've read numerous other posts with suggestions like leaving out spaces between commas, etc. but nothing works.

    Please help!

    I'm using Cufflinks-2.2.1 on Linux.

    My Code is:

    cuffdiff -p 12 -o ./cuffdiff/ -b /path/to/genome.fa –library-norm-method quartile -L TreatmentA,TreatmentB ./merged/merged/merged.gtf \
    ./cuffquant/Ctrl_1_abundances.cxb,./cuffquant/Ctrl_5_abundances.cxb,./cuffquant/Ctrl_9_abundances.cxb,./cuffquant/Ctrl_13_abundances.cxb \
    ./cuffquant/Sample_2_abundances.cxb,./cuffquant/Sample_6_abundances.cxb,./cuffquant/Sample_10_abundances.cxb,./cuffquant/Sample_14_abundances.cxb
    Thanks!!!
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Have you tried to run with "-v" (verbose) option to see if you get any additional diagnostic output?

    Comment

    • Sciurus
      Member
      • Dec 2013
      • 23

      #3
      Thanks for the suggestion!

      Strangely enough, I retyped the whole command and now it runs even though everything is still the same.

      Maybe there was some weird formation thing going on in my editor?

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        Always use a programmer's editor if you are editing the command line on a PC (Notepad++)/Mac(Textwrangler).

        Comment

        • Sciurus
          Member
          • Dec 2013
          • 23

          #5
          Yes, I am using Notepad++ but I have no other explanation for what went wrong before...

          Comment

          Latest Articles

          Collapse

          • kinpict.com
            Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by kinpict.com
            The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
            Today, 06:37 PM
          • SEQadmin2
            16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by SEQadmin2





            NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
            10-30-2026, 06:46 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 11-12-2026, 07:59 AM
          0 responses
          27 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 11-05-2026, 09:25 AM
          0 responses
          35 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 11-03-2026, 11:54 AM
          2 responses
          56 views
          0 reactions
          Last Post kinpict.com  
          Started by SEQadmin2, 10-28-2026, 09:57 AM
          0 responses
          175 views
          0 reactions
          Last Post SEQadmin2  
          Working...