Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Greg
    Member
    • Oct 2009
    • 31

    #1

    Can you spot whats wrong with this .gff3?

    Hello,
    I've been working with tophat for a while pretty successfully, but for some reason this one data set will not cooperate. Using the .gff file I downloaded for the corresponding genome I get:

    [Fri Jul 9 15:45:42 2010] Reading known junctions from GFF file
    Warning: TopHat did not find any junctions in GFF file
    I've tried making several adjustments to the file to make it fit the prescribed format. I've also double checked that the first column matches whats in the bowtie index using bowtie-inspect. Maybe someone else has had a similar experience? I've tried several variants on the following format and nothing helps, which makes me thing is probably something I've overlooked and I am out of ideas.

    Chr01 glean gene 47206323 47208329 . - . ID=BGIOSGA000006;Name=BGIOSGA000006;
    Chr01 glean mRNA 47206323 47208329 0.999993 - . ID=BGIOSGA000006;Name=BGIOSGA000006-TA;Parent=BGIOSGA000006;ID=BGIOSGA000006.50;Name=BGIOSGA000006-TA;ID=BGIOSGA000006;
    Chr01 glean exon 47208026 47208329 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.51;
    Chr01 glean exon 47207590 47207951 . - 2 Parent=BGIOSGA000006;ID=BGIOSGA000006.52;
    Chr01 glean exon 47207158 47207394 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.53;
    Chr01 glean exon 47206323 47206556 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.54;
    Thanks in advance.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
31 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
39 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
45 views
0 reactions
Last Post SEQadmin2  
Working...