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The 454 SFF tools will split data sets by MIDs and remove the MIDs as well. If you have a data set with MIDs at the 5'-end, then it only removes them from the 5'-end. If you have a data set with MIDs at both end, then it will remove both MIDs and also split accordingly. If not, contact the 454 support. -
spliting to mids- with mids on both sides
Hi.
I need help with splitting my reads to the samples they refer to, but each sample was tagged with a mid at the 5' end and at the 3' end . for example: sample 1 is tagged with mids 1 at the beginning and 2 at the end, and sample 2 was tagged with mid 1 at the beginning and mid 3 at the end etc. So I need to have reads with mid1-read-mid2 separated from the reads mid1-read-mid3 etc...
I saw you can split reads using sfffile, but I couldn't find there a way to solve this issue of mids at both ends. Thank you!!!!
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