Would this still be a useful tool to assess ChIP quality? If I understand correctly, this is used for single end experiments because it is necessary to estimate the fragment size (but unnecessary since paired-end data gives you exact fragment size).
Has anyone computed this anyway with paired-end data as a measure for how well your IP worked? If so, would simply using either R1 or R2 of each pair be a reasonable approach?
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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