Thank you very much for your website and all of your help. I am attempting to use IGV to look at a few genes in order to calculate the number of non-synonymous SNPs. However, there were large regions where the number of reads dropped from 10000 to as low as 500. If we find that there is a region with a high rate of nonsynonymous SNPs but it only contains an average of 500 reads at that point is that significant if other regions contain 5000 reads? In other words, if a single nucleotide shows 5% variability (eg 95% A and 5%T) with 500 reads is that similar to 5% variability at 5000 reads? Or do we need to treat the 4500 reads not showing up in IGV at that spot as potentially too dissimilar to run statistical analysis? Any help or references you could provide would be much appreciated.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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