Bowtie2 Issue:Backward call to driver()

[Milestailsprowe]

Hello everyone I'm trying to use bowtie2-build and keep getting backward call to driver for mirror index issue.

I'm not sure what the issue is

I'm using

bowtie2-build -f genome.fa mouse_build

on the Mus_musculus build from Igenome

[dpryan]

1. Can you post the exact error message?
2. You can probably find prebuilt indices on iGenomes.

[GenoMax]

@milestailsprowe: If you downloaded the tar bundle from iGenomes it already has pre-built indexes for multiple aligners (e.g. look in directories /path_to/Sequence/Bowtie2Index or Sequence/BowtieIndex (for bowtie v.1)).

[Milestailsprowe]

I retried bowtie2-build on a set I've gotten it to work and gor Total time for backward call to driver() for mirror index:1:31:06

All I did was

bowtie2-build (name of the index) and then (what I wanted it ot be called)

This is a whole new issue for me

[GenoMax]

bowtie2-build is used to "create" new indexes from a plain fasta sequence file. Why are you trying to run bowtie2-build on a pre-built index that is ready for use with tophat/bowtie2?

[Milestailsprowe]

bowtie2-build is used to "create" new indexes from a plain fasta sequence file. Why are you trying to run bowtie2-build on a pre-built index that is ready for use with tophat/bowtie2?

I got it to work doing that with Bowtie2-Align before it would say Bowtie2 exited at value 1. I got the Mouse one to to work from Igenome but now a set I've done Bowtie2-Build on before is giving me this issue with the same commands.