Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • wangxueqin
    Junior Member
    • Oct 2013
    • 2

    #1

    vcf convert to fasta, the fasta file is not an alingment file

    i have multiple samples. i used HaplotypeCaller in GATK like:
    java -jar GenomeAnalysisTK.jar -T HaplotypeCaller -R ./pe.scaffolds.db.fasta -I ./AEM.dedupped.bam -o ./AEM.raw_variants.g.vcf . --genotyping_mode DISCOVERY -stand_emit_conf 10 -stand_call_conf 30 --allow_potentially_misencoded_quality_scores --emitRefConfidence GVCF -variant_index_type LINEAR -variant_index_parameter 128000

    then i used GenotypeGVCFs to combine samples
    java -jar GenomeAnalysisTK.jar \
    -T GenotypeGVCFs \
    -R pe.scaffolds.db.fasta \
    --variant 123480.raw_variants.g.vcf \
    --variant 123651.raw_variants.g.vcf \
    -o 56species_raw.vcf \

    and, i used SelectVariants like this:
    java -jar GenomeAnalysisTK.jar \
    -T SelectVariants \
    -R pe.scaffolds.db.fasta \
    -V 56species_raw.vcf \
    -L PH01000000 \
    -ef \
    -o 56species_test.vcf

    i get the vcf files, then i used vcftools convert the vcf file to fasta file. but i found that in the fasta file, different samples have different number of loci. i want to do phylogenetic analysis, now, i do not know how to solve the problem.
  • wangxueqin
    Junior Member
    • Oct 2013
    • 2

    #2
    i used bowtie+samtools also, i converted the vcf file to fasta file. in the fasta file, evry samples had the same length. so i wonder may be i set the wrong parameters in GATK?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      Yesterday, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    71 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...