Why you don't use blast algorithm for read alignment?
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+ blast is just too slow, NGS mappers sacrify quality for speed. Try to align 1E6 50bp reads on a human genome...
+ blast doesn't handle paired-end sequencing, quality, ...
+ blast doesn't (officially) support the standard BAM format -
That's true (as are your other points), but a little misleading. Bear in mind that there are a lot of flavors of BLAST; some of them are designed to convert nucleotides to amino acids and map in protein space, which is not useful for noncoding reads. Also, I would argue that short-read aligners can give higher-quality output than BLAST, despite being optimized for speed. Consider RNA-seq data mapped to a genome, for example - appropriate NGS aligners would give much higher quality output compared to BLAST.Originally posted by lindenb View PostComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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