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  • dawn1313
    Member
    • Aug 2015
    • 21

    #1

    cuffmerge not work for all samples

    Hi,

    I am doing cuffmerge to merge the six assemblies. It worked well for the three samples together, but it has the following error when the total six assemblies in the assembly_list.txt.

    ---------------------------------------
    > cuffmerge -p 4 -g ../ref_annotation.gff3 -s CMV_ref.fa assembly_list.txt

    [Tue Sep 8 10:19:15 2015] Beginning transcriptome assembly merge
    -------------------------------------------

    [Tue Sep 8 10:19:15 2015] Preparing output location ./merged_asm/
    [Tue Sep 8 10:19:15 2015] Converting GTF files to SAM
    [10:19:15] Loading reference annotation.
    [10:19:15] Loading reference annotation.
    [10:19:15] Loading reference annotation.
    GFF Error: duplicate/invalid 'transcript' feature ID=UL122
    [FAILED]
    Error: could not execute gtf_to_sam

    ----------------------------------------
    Is there some help? Thanks a lot!
    Dawn

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