Hello all,
I have installed NCBI-Blast-2.2.28 on win-7 32 bit system .
Also set the path variable to bin directory
i want to create database of plant microRNAs
i have downloaded mature micoRNAs from miRBase
Right now i am having an excel file containing plant microRNas from miRBase
I want to convert this data in to blastable format
I have converted the excel file into csv format and then in to fasta format
and tried makeblastdb command , but it outputs BLAST options errorr: file doesn't exist
can you please help me regarding this
Thanks for your help in advance
I have installed NCBI-Blast-2.2.28 on win-7 32 bit system .
Also set the path variable to bin directory
i want to create database of plant microRNAs
i have downloaded mature micoRNAs from miRBase
Right now i am having an excel file containing plant microRNas from miRBase
I want to convert this data in to blastable format
I have converted the excel file into csv format and then in to fasta format
and tried makeblastdb command , but it outputs BLAST options errorr: file doesn't exist
can you please help me regarding this
Thanks for your help in advance