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  • jinu chirayath
    Junior Member
    • Jan 2015
    • 1

    #1

    BLAST + database creation for plant microRNA s from miRBase

    Hello all,

    I have installed NCBI-Blast-2.2.28 on win-7 32 bit system .
    Also set the path variable to bin directory
    i want to create database of plant microRNAs
    i have downloaded mature micoRNAs from miRBase
    Right now i am having an excel file containing plant microRNas from miRBase
    I want to convert this data in to blastable format
    I have converted the excel file into csv format and then in to fasta format
    and tried makeblastdb command , but it outputs BLAST options errorr: file doesn't exist
    can you please help me regarding this

    Thanks for your help in advance

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