I have a collapsed fasta files with all my counts and the corresponding sequences. I would like to annotate those sequences onto a gff file to find out what they are. Is this possible? Usually I would need a bam/sam file, but I dont have that for these sequences.
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So map the collapsed fasta file to something and then count accordingly. Just make the counts part of the read name so you can get them back later. BTW, you made your life more difficult by collapsing things like that. Aligners are fast enough these days to handle whatever size data you want to throw at them.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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