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  • repinementer
    Member
    • Dec 2009
    • 80

    #1

    tophat gff file error

    Code:
    tophat -r 20 hg18_c read1.gff read2.gff
    Error: file SL001_R00089_RHE011final_unique.gff does not appear to be a valid FASTA or FASTQ file

    Is there any way to convert gff to fastQ format?
    and How could I use -G option to read gff files in tophat?

    Thanx
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    Originally posted by repinementer View Post
    Is there any way to convert gff to fastQ format?
    No, that would be meaningless. GFF files are for features on a sequence (typically a chromosome or a contig), while FASTQ for sequences with base qualities (typically single reads).

    Comment

    • repinementer
      Member
      • Dec 2009
      • 80

      #3
      yes

      yes sorry my bad.

      Comment

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