Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • m_elena_bioinfo
    Member
    • Oct 2009
    • 99

    #1

    number of reads

    Hi users,
    I want to know number of reads from Illumina fastq (.txt file), number of reads that are aligned by bwa (from bam files), number of reads that are aligned in target genes (genes enrichment sequencing).

    Someone could help me?
    Thanx a lot!!!
    ME
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    What programming languages do you know (if any)? Or are you hoping for command line tool recommendations?

    Comment

    • m_elena_bioinfo
      Member
      • Oct 2009
      • 99

      #3
      I'm trying samtools idxstats for mapped reads and this command for reads in target genes
      > samtools view file.bam | awk '$3=="chr" && $4>=start_target_genes && $8<=end_target genes' > reads
      > wc -l reads

      Is this ok?

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      53 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      39 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      46 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      46 views
      0 reactions
      Last Post SEQadmin2  
      Working...