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  • FelipeAd
    Member
    • Jan 2011
    • 17

    #1

    GO functional annotation of sequences

    What is the best free software to annotate fasta sequences using GO terms?

    I am looking for a tool similar to blast2go.
  • Mocca
    Junior Member
    • Mar 2012
    • 9

    #2
    Have you checked out the Trinotate pipeline? Quite extensive, but provides a pretty good annotation. You could run just the GO annotation steps or the whole thing using blast, signalp... I also found that the Trinity/Trinotate folks have a topGO script (not extensively tested though). This might be useful as I spent a lot of time parsing the trinotate report into lists of GO:annotations which I further used in Cytoscape/BinGO. I work on a non-model vertebrate.

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