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  • Genom
    Junior Member
    • Jan 2022
    • 7

    #16
    BWA 2 files fasq to 1 fil sam

    I am creating a sam file, with 2 fastq files, using bwa
    I apply the following command

    bwa mem -t 2 GRCh38.primary_assembly.genome.fa.gz V350019555_L03_B5GHUMqcnrRAABA-556_1.fq.gz
    V350019555_L03_B5GHUMqcnrRAABA-556_2.fq.gz
    > V350019555_L03_B5GHUMqcnrRAABA-556.sam


    The alignment begins to be generated, but after 15 hours, the following appears, and it generates a 0-bit sam file


    V350019555L3C006R0051051033 16 chr21 9651911 29 150M * TGTTTTCTCTTTTTACAGCAAGGACAGTTTATTAAACACACTGTTTTAAACCTTATTTTTTCTCTAAATAATATTTCATATAAGTCAATATGTAGACTTTATGTATTTTTAAAATGAAGACATAGCATTCCATTGAACTTAGACAAAAAT EFFFFFFEGFFFFGEFF9FEFFFFGGFCFFFFGFFFFFFDFFFFFFFFFFFFFGAFFFCGBFDF@G@FGFFGFGFFFFGFFFFGFGFCFFFFFFFDGFGFGFFGFFFFFAFFFFFFEFFFFFFFFFFGDFFFF@FG8FF?FFFFFFGFFF NM:i:1 MD:Z:23G126 AS:i:145 XS:i:131 XA:Z:chr4,+189704487,150M,4;
    [main] Version: 0.7.17-r1188
    [main] CMD: bwa mem -t 2 GRCh38.primary_assembly.genome.fa.gz V350019555_L03_B5GHUMqcnrRAABA-556_1.fq.gz
    [main] Real time: 26995.284 sec; CPU: 33394.341 sec
    zsh: command not found: V350019555_L03_B5GHUMqcnrRAABA-556_2.fq.gz





    I have previously analyzed the files with fasqc, and they seem correct


    What is happening, what should I do?

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #17
      @Genom: As answered on Biostars you need to have all command line options on one line. If command needs to span multiple lines then a \ is needed at the end of incomplete lines.

      Comment

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