Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Anna Esteve
    Junior Member
    • Jan 2010
    • 7

    Cufflinks, attribute not supported

    Hi,
    I run Cufflinks with sam input (from Tophat) and I got this error message thousands of times:
    'NH attribute not supported'
    Does anyone know how to solve this problem?
    Thanks in advance.
  • sdriscoll
    I like code
    • Sep 2009
    • 436

    #2
    Is Cufflinks still generating output along with these errors or are you only getting errors? I've seen some errors from Cufflinks in the past but it didn't get in the way of it generating otherwise normal looking output. It sounds like something in the SAM file that Cufflinks doesn't like though that doesn't make much sense.
    /* Shawn Driscoll, Gene Expression Laboratory, Pfaff
    Salk Institute for Biological Studies, La Jolla, CA, USA */

    Comment

    • Anna Esteve
      Junior Member
      • Jan 2010
      • 7

      #3
      Cufflinks reports that doesn't support NH, CC and CP attributes. So I had to delete all this attributes from my Sam file and now I think is working fine.

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 11:08 AM
      0 responses
      6 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      11 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      19 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      53 views
      0 reactions
      Last Post SEQadmin2  
      Working...