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  • cmccabe
    Senior Member
    • Jul 2012
    • 355

    #1

    bbduk and kmer masking

    Using bbduk on Illumina PE hg19 fastq files from NextSeq. I have done adapter removal, quality trimming and contamination filtering. Is kmer masking needed or recommended before I align using bbmap? Thank you .
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    Originally posted by cmccabe View Post
    Using bbduk on Illumina PE hg19 fastq files from NextSeq. I have done adapter removal, quality trimming and contamination filtering. Is kmer masking needed or recommended before I align using bbmap? Thank you .
    Nope! That's just for special use-cases.

    Comment

    • cmccabe
      Senior Member
      • Jul 2012
      • 355

      #3
      Thank you .

      The bbMap tools are amazingly fast and accurate.

      Comment

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