Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • cmccabe
    Senior Member
    • Jul 2012
    • 355

    #1

    picard 1.139 error trying to index bam

    I do not understand the eroor recieved from picard just trying to index a bam. Why does the output path get duplicated? It also seems to happen in mark as duplicates. Thank you .

    Code:
    cmccabe@DTV-A5211QLM:~/Desktop/NGS$ java -jar /home/cmccabe/Desktop/NGS/picard-tools-1.139/picard.jar BuildBamIndex \
    > INPUT=home/cmccabe/Desktop/NGS/picard-tools-1.139/dedup_sorted_mapped_unmatched_adapter_removed_quality_trimmed_NA12878-NextSeq-S1_R1_and_R2.bam
    [Wed Nov 04 09:32:11 CST 2015] picard.sam.BuildBamIndex INPUT=home/cmccabe/Desktop/NGS/picard-tools-1.139/dedup_sorted_mapped_unmatched_adapter_removed_quality_trimmed_NA12878-NextSeq-S1_R1_and_R2.bam    VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json
    [Wed Nov 04 09:32:11 CST 2015] Executing as cmccabe@DTV-A5211QLM on Linux 3.19.0-31-generic amd64; OpenJDK 64-Bit Server VM 1.7.0_85-b01; Picard version: 1.139(8ceee52414e8ab9d13e350ff9cd86d48825dd64d_1442240108) IntelDeflater
    [Wed Nov 04 09:32:11 CST 2015] picard.sam.BuildBamIndex done. Elapsed time: 0.00 minutes.
    Runtime.totalMemory()=883425280
    To get help, see http://broadinstitute.github.io/picard/index.html#GettingHelp
    Exception in thread "main" htsjdk.samtools.SAMException: [B]Cannot write file: /home/cmccabe/Desktop/NGS/home/cmccabe/Desktop/NGS/picard-tools-1.139/dedup_sorted_mapped_unmatched_adapter_removed_quality_trimmed_NA12878-NextSeq-S1_R1_and_R2.bai. Neither file nor parent directory exist.[/B]
    	at htsjdk.samtools.util.IOUtil.assertFileIsWritable(IOUtil.java:371)
    	at picard.sam.BuildBamIndex.doWork(BuildBamIndex.java:113)
    	at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:206)
    	at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:95)
    	at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:105)
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    You probably forgot a "/" in "INPUT=home/..." (try "INPUT=/home/...").

    Comment

    • cmccabe
      Senior Member
      • Jul 2012
      • 355

      #3
      That was it... thank you .

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      21 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      18 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      42 views
      0 reactions
      Last Post SEQadmin2  
      Working...