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  • Genotype association help

    Dear All,

    Please forgive my ignorance I am new to association studies. Please provide me with some comments and insight on what I would like to achieve.

    Maim goal:
    I have genotyped 100+ individuals for 40 SNP makers and would like to associate a genotype with particular phenotypic trait etc.

    Phenotypes:
    1. I have several phenotype traits I would like to associate a genotype with.
    2. The individuals are also group into different class based on several phenotype observations.
    What I have read so far is that I can add phenotypic trait to a phenotype file to be used with plink. With regards to the groups do I treat each group as a "phenotype" and also add these to the phenotype file I will be using with plink.

    Results
    What I would like to achieve in the end is for each group a representative genotype. Would this be possible any help would be appreciated, if possible examples of ped,map and pheno files would also be appreciated as this is what I am aslo unsure of as to how to represent this is the different files.

    Thanks

  • #2
    Any insight help would be appreciated.

    Have I posted in the wrong section?
    Last edited by Altair; 11-09-2015, 09:34 PM.

    Comment


    • #3
      A genome-wide association study using 40 SNPs is almost completely useless, and very likely to net useless false positive associations when testing against a large array of phenotypes.

      Comment


      • #4
        Thanks for the response.
        I do believe I am not expressing myself clearly I apologies.
        The 40SNPs that have been chosen have been associated with the phenotypes/traits in previous studies (published) so the association to the phenotype/trait does indeed exist. I would like to determine if a specific SNP call ( A/G or A/C ) has a effect on the trait/phenotype.

        Example:
        These are just to explain what I would like to achieve and not real cases.
        Using on 2 markers for this example and 8 plants (same species).

        Marker 1 : associated with flower colour
        Marker 2 : associated with plant height
        Plant 1 Flower colour : Red
        Plant 2 Flower colour : Yellow
        Plant 3 Flower colour: Red
        Plant 4 Flower colour : Yellow
        Plant 1 height : 60cm (classified as tall)
        Plant 2 height : 45cm (classified as average)
        Plant 3 height : 52cm (classified as average)
        Plant 4 height : 62cm (classified as tall )
        So results for
        Plant Marker 1 Maker 2
        1 A/A C/A
        2 A/T C/T
        3 A/A C/T
        4 A/T C/A
        Given the example results if a seedling of a plant is test for bother markers and Marker 1 is A/A is could be hypothesized that it will have red flowers and Marker 2 is C/T it would be of average height.

        I do hope this helps.

        Thanks

        Comment

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