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  • akvarel
    Member
    • Nov 2011
    • 11

    GFOLD posterior distribution

    It seems I do not understand the posterior distribution described in this paper:




    For a gene, given the observed read counts under two condi- tions, the posterior distribution of expression levels x1 and x2 under the two conditions can be calculated as above.


    What values does lambda take in the formula of Post(lambda)? Does it take values from 0 to 1000, for example? And for each of this values a posterior probability is calculated and the most probable lambda is taken?

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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