How to have a library of nucleic acid sequences without going on NCBI every time ?
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Without knowing the full context of this question here are some general suggestions.
You could keep blast indexes for "nt"(since you asked for nucleic acids) database handy and extract sequences you need using blastdbcmd utility.
You could also use e-utils from NCBI and fetch data you need (but this can count indirectly as "going on NCBI").Last edited by GenoMax; 12-01-2015, 05:59 PM.
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You are going to have to visit some websites ....
If you are working with eukaryotic genes NCBI may have already done the work for you in their homologene project in terms of multiple sequence alignments. Ensembl and UCSC also have similar results available.
On prokaryotic side you can check this site: http://mbgd.genome.ad.jp/
If you only have accession numbers then you may need to do some alignments yourself.
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