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  • szy0931
    Member
    • Aug 2015
    • 51

    #1

    cummeRbund cannot read cuffdiff results

    I ran cuffdiff on Galaxy and downloaded the resulting .tabular files.
    However, cummeRbund on my computer (windows 10, R environment) cannot open the .tabular files.
    How to change the tabular format to a format that can bed opened by cummeRbund?
    Thank you very much!
  • blancha
    Senior Member
    • May 2013
    • 367

    #2
    Have you tried the function readCufflinks?
    If not, that would be your first step.
    If you have already tried, what was the error message, and what is your session info?
    sessionInfo()

    Comment

    • szy0931
      Member
      • Aug 2015
      • 51

      #3
      Originally posted by blancha View Post
      Have you tried the function readCufflinks?
      If not, that would be your first step.
      If you have already tried, what was the error message, and what is your session info?
      sessionInfo()

      I just ran like this

      > setwd("C:/Users/szy0931/Desktop/A")
      > library(cummeRbund)
      > cuff=readCufflinks(dbFile="cuffData.db",
      + splicing="581",
      + promoters="582",
      + CDSoverloading="583",
      + CDSFPKMdifferential="584",
      + CDSFPKMtracking="585",
      + TSSgroupsdifferential="586",
      + TSSgroupsFPKMtracking="587",
      + genedifferential="588",
      + geneFPKM="589",
      + transcriptdifferential="590",
      + transcriptFPKM="591",
      + rebuild=T)

      Here:
      581-591 are the names I renamed the tabular files I downloaded from galaxy
      581 is: Galaxy581-[Cuffdiff_on_data_519,_data_514,_and_others__splicing_differential_expression_testing]
      582 is: Galaxy582-[Cuffdiff_on_data_519,_data_514,_and_others__promoters_differential_expression_testing]
      583 is:Galaxy583-[Cuffdiff_on_data_519,_data_514,_and_others__CDS_overloading_diffential_expression_testing]
      584 is:Galaxy584-[Cuffdiff_on_data_519,_data_514,_and_others__CDS_FPKM_differential_expression_testing]
      585 is: Galaxy585-[Cuffdiff_on_data_519,_data_514,_and_others__CDS_FPKM_tracking]
      586 is: Galaxy586-[Cuffdiff_on_data_519,_data_514,_and_others__TSS_groups_differential_expression_testing]
      587 is: Galaxy587-[Cuffdiff_on_data_519,_data_514,_and_others__TSS_groups_FPKM_tracking]
      588 is: Galaxy588-[Cuffdiff_on_data_519,_data_514,_and_others__gene_differential_expression_testing]
      589 is:Galaxy589-[Cuffdiff_on_data_519,_data_514,_and_others__gene_FPKM_tracking]
      590 is: Galaxy590-[Cuffdiff_on_data_519,_data_514,_and_others__transcript_differential_expression_testing]
      591 is:Galaxy591-[Cuffdiff_on_data_519,_data_514,_and_others__transcript_FPKM_tracking]

      The file "Galaxy592-[Cuffdiff_on_data_519,_data_514,_and_others__cummeRbund_SQLite_database]" was also downloaded.

      I got the following:
      Creating database C:/Users/szy0931/Desktop/A/cuffData.db
      Reading C:/Users/szy0931/Desktop/A/589
      Error in file(file, "rt") : cannot open the connection
      In addition: Warning message:
      In file(file, "rt") :
      cannot open file 'C:/Users/szy0931/Desktop/A/589': No such file or directory

      It looks 589 has some problems.

      I then ran
      > list.files()
      I got this:
      [1] "581.tabular" "582.tabular" "583.tabular" "584.tabular" "585.tabular" "586.tabular" "587.tabular" "588.tabular" "589.tabular" "590.tabular" "591.tabular"
      [12] "592.sqlite" "cuffData.db"
      So, it means 589 is here, right?

      I ran sessionInfo()
      > sessionInfo()
      I got:
      Attached Files
      Last edited by szy0931; 12-02-2015, 03:28 PM.

      Comment

      • szy0931
        Member
        • Aug 2015
        • 51

        #4
        figured out. I used run names. I am a totally idiot.

        Comment

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