Hello,
I am running Tophat with the --GFF option, where I supply it with the GFF of all the genes in my genome so that it can use those existing junctions in addition to discovering novel ones.
Two questions:
1. After the Tophat run, how can I check that Tophat actually used the junctions I gave it? Is there a .log file I can check? I cannot seem to find anything about the status of those junctions in "run.log".
2. Will Tophat make combinatorial use of the junctions I give it? In other words, if we have a gene with a single mRNA comprised of three exons, will it consider the junction where the middle exon is skipped, or does it only consider the junctions explicit in the GFF?
Thanks.
I am running Tophat with the --GFF option, where I supply it with the GFF of all the genes in my genome so that it can use those existing junctions in addition to discovering novel ones.
Two questions:
1. After the Tophat run, how can I check that Tophat actually used the junctions I gave it? Is there a .log file I can check? I cannot seem to find anything about the status of those junctions in "run.log".
2. Will Tophat make combinatorial use of the junctions I give it? In other words, if we have a gene with a single mRNA comprised of three exons, will it consider the junction where the middle exon is skipped, or does it only consider the junctions explicit in the GFF?
Thanks.